A box for PCR diagnostics

Purpose and Contamination Control

The PCR cabinet is designed to prevent intra-laboratory contamination during polymerase chain reaction (PCR) procedures. It provides a localized sterile zone in medical and research laboratories for operations involving DNA and RNA, where the exclusion of cross-contamination between samples is critical for diagnostic accuracy.

Operating Principle and Decontamination

The cabinet is equipped with a directed airflow system and germicidal ultraviolet (UV) lamps for the treatment of internal surfaces, instruments, and consumables. UV irradiation effectively neutralizes DNA contaminants, thereby preventing false-positive results in PCR analysis.

Construction and Safety Features

The enclosure is fabricated from materials resistant to disinfectants and long-term UV exposure. The working chamber is engineered with optimal ergonomics and integrated lighting to ensure a comfortable and safe working environment. The design includes specialized shielding to protect the operator from direct UV radiation during the decontamination cycle.

Compliance and Validation

PCR cabinets comply with GMP, GLP, and relevant sanitary standards. Validation procedures, including the qualification of disinfection systems and airflow patterns, are performed in alignment with GMP Annex 11 and GAMP 5 recommendations to ensure process reliability and data integrity.